Making bacterial stock solution:27-10-2022

Realisation:28-10-2022

The strain will be fresh to be inoculated and then frozen so E.coli and S.aureus must be renewed 24 hour before and then inoculated in skim milk before freezing at -20. 

Solution preparation

NaOH(1M):

- Fill the flask half way with distilled water

- Add 40g of NaOH powder

-Add water to reach the 1L mark

-finally mix it to obtain NaOH solution (1M).

H₂SO₄(1M):

-Fill the flask half way with distilled water

Add 56 ml of H₂SO₄

-add water to reach the 1L mark

-Finally mix it to obtain H₂SO₄ solution (1M).

NaCl 0.9%:

-We put 0.9g NaCl in 100 ml distilled water

-Put it in Autoclave for microbial usage. 

Bleach 1%:

-We took 1ml of bleach solution

-Put it in 100ml water graduated cylinder

-Mix it to obtain Bleach solution 1%.

Muller Hinton agar:

-Measure 5.7g of Muller Hinton powder

-Put it in 150 ml of distilled water

-Mix them on magnetic stirrer with heating (to reach boiling point)

-Put it in Autoclave (1h) for microbial usage.

Standard agar: 

-Measure 5.55g of standard agar powder

-Put it in 150 ml of distilled water

-Mix them on magnetic stirrer with heating (to reach boiling point)

-Put it in Autoclave (1h) for microbial usage.

Broth: 

 -Measure 5g peptone, 1g tryptone, 1g glucose, 1g yeast extract and 2.5g sodium chloride

-Put it in 500ml of distilled water

-Mix them on magnetic stirrer with heating (to reach boiling point)

-Put it in Autoclave (1h) for microbial usage.

Phosphate buffer (0.1M, pH:7):

 -Prepare 800 ml of distilled water in a suitable beaker

-Add 9.34g of Potassium phosphate dibasic (0.05364M, mw: 174.18g/mol) to the solution

-Add 6.31g of Potassium phosphate monobasic (0.0436M, mw: 136.09 g/mol) to the solution

-Add water until the volume reach 1L

-Put it in Autoclave (1h) for microbial usage.

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