Solution preparation

NaOH(1M):

- Fill the flask half way with distilled water

- Add 40g of NaOH powder

-Add water to reach the 1L mark

-finally mix it to obtain NaOH solution (1M).

H₂SO₄(1M):

-Fill the flask half way with distilled water

Add 56 ml of H₂SO₄

-add water to reach the 1L mark

-Finally mix it to obtain H₂SO₄ solution (1M).

NaCl 0.9%:

-We put 0.9g NaCl in 100 ml distilled water

-Put it in Autoclave for microbial usage. 

Bleach 1%:

-We took 1ml of bleach solution

-Put it in 100ml water graduated cylinder

-Mix it to obtain Bleach solution 1%.

Muller Hinton agar:

-Measure 5.7g of Muller Hinton powder

-Put it in 150 ml of distilled water

-Mix them on magnetic stirrer with heating (to reach boiling point)

-Put it in Autoclave (1h) for microbial usage.

Standard agar: 

-Measure 5.55g of standard agar powder

-Put it in 150 ml of distilled water

-Mix them on magnetic stirrer with heating (to reach boiling point)

-Put it in Autoclave (1h) for microbial usage.

Phosphate buffer (0.1M, pH:7):

 -Prepare 800 ml of distilled water in a suitable beaker

-Add 9.34g of Potassium phosphate dibasic (0.05364M, mw: 174.18g/mol) to the solution

-Add 6.31g of Potassium phosphate monobasic (0.0436M, mw: 136.09 g/mol) to the solution

-Add water until the volume reach 1L

-Put it in Autoclave (1h) for microbial usage.