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The most important solutions in biology lab
Solution preparation
NaOH(1M):
- Fill the flask half way with distilled water
- Add 40g of NaOH powder
-Add water to reach the 1L mark
-finally mix it to obtain NaOH solution (1M).
H₂SO₄(1M):
-Fill the flask half way with distilled water
Add 56 ml of H₂SO₄
-add water to reach the 1L mark
-Finally mix it to obtain H₂SO₄ solution (1M).
NaCl 0.9%:
-We put 0.9g NaCl in 100 ml distilled water
-Put it in Autoclave for microbial usage.
Bleach 1%:
-We took 1ml of bleach solution
-Put it in 100ml water graduated cylinder
-Mix it to obtain Bleach solution 1%.
Muller Hinton agar:
-Measure 5.7g of Muller Hinton powder
-Put it in 150 ml of distilled water
-Mix them on magnetic stirrer with heating (to reach boiling point)
-Put it in Autoclave (1h) for microbial usage.
Standard agar:
-Measure 5.55g of standard agar powder
-Put it in 150 ml of distilled water
-Mix them on magnetic stirrer with heating (to reach boiling point)
-Put it in Autoclave (1h) for microbial usage.
Phosphate buffer (0.1M, pH:7):
-Prepare 800 ml of distilled water in a suitable beaker
-Add 9.34g of Potassium phosphate dibasic (0.05364M, mw: 174.18g/mol) to the solution
-Add 6.31g of Potassium phosphate monobasic (0.0436M, mw: 136.09 g/mol) to the solution
-Add water until the volume reach 1L
-Put it in Autoclave (1h) for microbial usage.