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Pilot scale system test



Step Description

Expected Result


System is off


open the valves   

open the valves from the control panel

Release the air from the system  

Switch on the system


Turn on the system from the control panel


THE SYSTEM IS heating the water till reach 121°c

Sterilization the fermenter

Open the fermenter valve

The steam is filling the fermenter

Sterilization the whole system

Open the last valve to sterilize last 2 tanks  

Pressure should reach 2 atm and temperature 121 °c

Open the exit pipe

Operate the exit pipe to open through the control panel

Pressure should decrease and the steam vent off the system


System off the system

 system is off


1. Turning on the systeme through the Control panel till reaching the expected temperature and pressure.

2. Opening the Valves (fermenter and purification tanks) to complete the sterilization test.

3. Opening the manual valve to insure the steam is exist. 


video autoclave test part 1

Video autoclave test part 2