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002:WHOLE SYSTEME TEST (AUTOCLAVE TEST)
Step |
Step Description |
Results |
Precondition |
System is OFF |
|
open/close the valves |
open all valves from the control panel and reclosed them after few minutes |
Release the air from the system |
Switch on the heater |
Turn on the heater from the control panel |
THE SYSTEM IS heating the water till reach 134°C |
Sterilization of the fermenter |
Open the autoclave valve (autolave--->Autoclave valve) |
The steam is filling the fermenter |
Sterilization of the whole system |
Open the fermenter valve to sterilize last 2 tanks (autolave--->fermenter--->fermenter valve) |
Pressure should reach 2 atm (20000 Pa) and temperature 134 °C |
Open the manual discharge valve |
Open the discharge valve manually |
Pressure should decrease and the steam vent off the system |
CLose the valves |
close ALL the valves from the control panel and the discharge valve manually |
ALL the valves are closed |
switching OFF the system |
switch off the system |
the system is OFF |
Postcondition |
system is OFF |
1. Turning on the systeme through the Control panel till reaching the expected temperature and pressure.
2. Opening the Valves (fermenter and purification tanks) to complete the sterilization test.
3. Opening the manual valve to insure the steam is exist.