002:WHOLE SYSTEME TEST (AUTOCLAVE TEST)

Step	Step Description	Results
Precondition	System is OFF
open/close the valves	open all valves from the control panel and reclosed them after few minutes	Release the air from the system
Switch on the heater	Turn on the heater from the control panel	THE SYSTEM IS heating the water till reach 134°C
Sterilization of the fermenter	Open the autoclave valve (autolave--->Autoclave valve)	The steam is filling the fermenter
Sterilization of the whole system	Open the fermenter valve to sterilize last 2 tanks (autolave--->fermenter--->fermenter valve)	Pressure should reach 2 atm (20000 Pa) and temperature 134 °C
Open the manual discharge valve	Open the discharge valve manually	Pressure should decrease and the steam vent off the system
CLose the valves	close ALL the valves from the control panel and the discharge valve manually	ALL the valves are closed
switching OFF the system	switch off the system	the system is OFF
Postcondition	system is OFF

1. Turning on the systeme through the Control panel till reaching the expected temperature and pressure.

2. Opening the Valves (fermenter and purification tanks) to complete the sterilization test.

3. Opening the manual valve to insure the steam is exist.