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MEGBI Planning&Control 2022

Lab contents 2022

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Report for July -Sep 2022 (Achieved)

Penicillin production:

Not achieved yet

Problems: conditions not respected (optimum temperature: 26-28°c, pH control: pH meter not working, shaker for the incubator and last edit: replace milk powder by lactose).

Penicillin quantification:

Crystallization not achieved penicillin G sodium, solvent need to changed butyl acetate instead of ethyl acetate achieved.   

Ampicillin production:

Achieved, but yield is low (concentration between: 0.17-0.20). 

Ampicillin quantification:

Crystallization not achieved ampicillin sodium, solvent need to changed butyl acetate instead of ethyl acetate.  

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MEGBI procurement list 2022

TODOs 2022:

    • Make sure of the buyed Penicillin G as Benzathin Penicillin G (2d, Material Costs: …)
    • Quantification of the produced Penicillin G (as Master Thesis)
    • Run the bioreactor in term to produce Penicillin G as pilot plant lab scale (as Master Thesis)
    • Production of Ampicillin from the produced penicillin G (as Master Thesis)
    • Quantification of the produced ampicillin (as Master Thesis)
    • Aspirin production Labscale (time: 2w, staff: 1MM, material costs: 50$) (planned: Feb 2020)

070122-MEGBI TODOs 2022 

Actions and Decisions List-MEGBI_16/5/2022 (xlsx)

Section TODO(s) Item Remarks Responsible(s) Due Date Status
(open/in work/done)		 Notes 
Penicillin production & quantification   penicillium cultivation in tryptone agar incubation for 4 days Hind 16/5/2022 done  
Penicillium cultivation in liquid medium incubation for 1 week Hind 20/5/2022 done  
Muller-Hilton Agar تحضير      30.5.22 done  
standard antibiotics preparation (disc)       1.6.22 done  

trial & real quantification test

     3.6.22 done  real quantification is stilled

real quantification test

 weigh obtained penicillin then the quantification will be applicable   8.8.22 done  

Find another protocol for production 

     10-14.6.22 done  

subculture of penicillium chrysogenum

     14.6.22 done  

culture of penicillium in liquid medium 

lab scale penicillin production 

9.8.22: in progress, for first time precursor (phenylacetate) ->penicillin G

 

 Mohammad, Jana 9.8.22 in work

delay due to precursor delivery (26.7.22)

P. cry. strain from doctoral school, LU Tripoli

test for the presence of penicillin

     15.8.22 open  

quantification of penicillin G

     16.8.22 open   

amp production from self-made penicillin & quantification

     about 15.9.22 open waiting for butyl-acetate (as solvent for penicillin purification)

step powderization of amp

 butyl-acetate as solvent for penicillin purification     open  
Ampicillin  production & quantification    E.coli and staph aureus culture (amp quantification) incubation for 24 hours Hind 16/5/2022 done  
Making Antibiotic standard solution (amp quantification)   Hind 18/5/2022 done Problem solved
Making Muller-Hinton agar+ autoclave (amp quantification)   Hind 18/5/2022 done  
Autoclave of distilled water, NaCl0.9% and materials required (amp quantification)     18/5/2022 done  
preparation of the standard 0.5MF (amp quantification)     18/5/2022 done  
6APA-R -> 6APA-ester (amp production with buyed penicillin G) (phosphate buffer (to be delivered from Germany on 8./9.6.22)  1 month    10.6.-26.7.22 done  done in July 22 (see rapport de stage (Alaa Bzal)), step powderization of amp still faced problems
amp quantification (all materials already prepared)  1 week   4.8.22 done  
Testing the effectiveness of our  presented ampicillin 2 days   6-7/6/2022 done  
testing the buyed ampicillin with our E.coli strain     1.7.22 done  
Explain and prepare the ampicillin production protocol     1.7.22 done  
Documentation      Whole study of requirements for large scale       done  
additional tasks into the report       done  
           
           
           
           
Pilot plant production     disc stack centrifuges for penicillin downstream processing  see at Jamal&Chaban    15.8.22  open  about 5800 $ (oil-water separation) (alibaba),