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MEGBI Planning 2022
TODOs 2022:
-
- Make sure of the buyed Penicillin G as Benzathin Penicillin G (2d, Material Costs: …)
- Quantification of the produced Penicillin G (as Master Thesis)
- Run the bioreactor in term to produce Penicillin G as pilot plant lab scale (as Master Thesis)
- Production of Ampicillin from the produced penicillin G (as Master Thesis)
- Quantification of the produced ampicillin (as Master Thesis)
- Aspirin production Labscale (time: 2w, staff: 1MM, material costs: 50$) (planned: Feb 2020)
Actions and Decisions List-MEGBI_16/5/2022 (xlsx)
| Section | TODO(s) Item | Remarks | Responsible(s) | Due Date | Status (open/in work/done) |
Notes |
| Penicillin production & quantification | penicillium cultivation in tryptone agar | incubation for 4 days | Hind | 16/5/2022 | done | |
| Penicillium cultivation in liquid medium | incubation for 1 week | Hind | 20/5/2022 | done | ||
| Muller-Hilton Agar تحضير | 30.5.22 | done | ||||
| standard antibiotics preparation (disc) | 1.6.22 | done | ||||
|
trial & real quantification test |
3.6.22 | done | real quantification is stilled | |||
|
real quantification test |
weigh obtained penicillin then the quantification will be applicable | 8.8.22 | done | |||
|
Find another protocol for production |
10-14.6.22 | done | ||||
|
subculture of penicillium chrysogenum |
14.6.22 | done | ||||
|
culture of penicillium in liquid medium |
17.6.22 | open | ||||
| Ampicillin production & quantification | E.coli and staph aureus culture (amp quantification) | incubation for 24 hours | Hind | 16/5/2022 | done | |
| Making Antibiotic standard solution (amp quantification) | Hind | 18/5/2022 | done | Problem solved | ||
| Making Muller-Hinton agar+ autoclave (amp quantification) | Hind | 18/5/2022 | done | |||
| Autoclave of distilled water, NaCl0.9% and materials required (amp quantification) | 18/5/2022 | done | ||||
| preparation of the standard 0.5MF (amp quantification) | 18/5/2022 | done | ||||
| 6APA-R -> 6APA-ester (amp production with buyed penicillin G) (phosphate buffer (to be delivered from Germany on 8./9.6.22) | 1 month | 10.6.-30.6.22 | open | |||
| (amp quantification) (all materials already prepared) | 1 week | 1.7.-5.7.22 | open | |||
| Testing the effectiveness of our presented ampicillin | 2 days | 6-7/6/2022 | done | |||
| testing the buyed ampicillin into our E.coli strain | 15.6.22 | open | ||||
| Explain and prepare the ampicillin production protocol | 15.6.22 | open | ||||
| Documentation | Whole study of requirements for large scale | open | ||||
| additional tasks into the report | done | |||||
| Pilot plant production | ||||||