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Biobutanol production
2 medias are possible (were used in the Paula et. al., "A two-phase Model for ABE Fermentation with a Modified Clostridium Acetobutylicum Strain", CEt, VOL. 110, 2024):
-1st) RCM uses Yeast
extract 3.0 g/L, Peptone from casein (Tryptone) 10.0 g/L, Meat extract 10.0 g/L, Glucose 5.0 g/L, Starch
-2nd)For
T6 media is used: Potassium dihydrogen phosphate 0.5 g/L, Magnesium sulphate heptahydrate 0.3 g/L, Iron
(II) sulphate heptahydrate 0.01 g/L, Ammonium acetate 3.0 g/L, Yeast extract 2.0 g/L, Peptone from casein
(Tryptone) 6.0 g/L, L-Cysteine hydrochloride 0.5 g/L, Agar 0.5 g/L and Glucose 40.0 g/L.
The first medium was used for culturing both strains and observing their grow for later fermentation
The second medium was used for fermentation.